Author(s):

Saravanan Devarajan, Gopiesh Khanna Venkatesan, Ashok Kumar Krishna Kumar and Vivek Pazhamalai

Abstract: ‘Red rust’ disease in tea plants was caused by the green alga Cephaleuros parasiticus Karst. It grows as an endophyte in tea plants which causes severe destruction to leaves than stem regions. The objective of this work was to analyze different pathogen-related (PR) enzymatic secretion by the plant and the pathogen amid the illness assault. A total of two isolates and one standard culture of Cephaleuros parasiticus namely VCP27, MCP11, and UTEX2412 was used in this study. Carbohydrases, defensive enzymes, phenolic enzymes, and oxidative enzymes were studied with various incubation periods. All the enzymes exhibited the highest activity at 30 min of the incubation period, however, CMCase of carbohydrases has shown optimal activity at 60 min of incubation. The study has shown that C. parasiticus was adept of generating numerous pathogenic enzymes which demonstrated a maximal activity at 30^th day harvest with the exception of protease enzyme which reveals the optimal activity at 40^th day of harvest. The above-mentioned enzyme activity was studied both in non-infected and red rust disease infected tea leaves. These findings confirm the C. parasiticus capability of producing different enzymes such as carbohydrases, pectinase, protease, chitinase and guarded chemicals alongside phenolic and oxidative proteins. Based on the enzyme activity, the harmfulness of the pathogen was discovered and discussed. The study is very much useful to understand the host-pathogen interaction and to produce the red rust disease resistant crop.

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