Journal of Pharmacognosy and Phytochemistry
Vol. 8, Issue 1 (2019)
Effect of hygromycin on peanut germination and its application in Agrobacterium-mediated genetic transformation
Amareshwari P, Venkatesh K and Roja Rani A
To overcome the laborious screening tasks in in planta
infected seed parts mainly embryonated cotyledons and embryos
can be passed through the selection of antibiotics for a very short duration for successful identification of transformants. Hygromycin B more toxic than the existing antibiotics and has been used as a selective agent in many crop plants. With the increase in hygromycin concentrations, a decline in the survival frequency of explants was observed. The threshold concentration of hygromycin for the selection of transformants was optimized using embryonated cotyledon and embryo on MS media containing different concentrations of hygromycin. It was observed that 20 mg/l hygromycin was threshold concentration for the selection of embryonated cotyledons and 15 mg/l for embryos. These concentrations suppress the growth of peanut plants. Therefore, hygromycin at 20 mg/l for embryonated cotyledons and embryos at 15 mg/l could screen hygromycin-tolerant transgenic peanuts.
Pages: 263-267 | 1226 Views 382 Downloads
Amareshwari P, Venkatesh K and Roja Rani A. Effect of hygromycin on peanut germination and its application in Agrobacterium-mediated genetic transformation. J Pharmacogn Phytochem 2019;8(1):263-267.