Journal of Pharmacognosy and Phytochemistry
Vol. 8, Issue 1 (2019)
Diversity and biological activities of fungal root endophytes of Hemidesmus indicus (L.) R. Br.
Shobha M, Bharathi TR, Sampath Kumara KK and Prakash HS
The roots of Hemidesmus indicus
are known to have bioactive properties. Large scale exploitation of plant roots may endanger the plant from ecosystem. Endophytes are known to produce many bioactive compounds as that of host plant. Endophytic fungi residing in various parts of the plant like root, stem and leaves were isolated and identified. Colonization frequency and relative dominance of the endophytes were evaluated. The ethyl acetate extracts of culture broth and mycelial mat was screened for antioxidant and antimicrobial activity.285 fungal isolates from the roots of H. indicus
identified and authenticated by molecular tools. The overall colonization frequency was found to be 70.33%, of which hyphomycetes were dominant (79.45%). Species of Fusarium
were the dominant colonizers of all parts of the plant. Antioxidant activities of ethyl acetate fractions revealed that the extracts of F. oxysporum
and A. sydowii
showed higher activities with IC50
values of 45 and 46.6 µg/mL respectively. The extracts also exhibited promising antibacterial activities against both Gram positive and Gram negative bacteria. The present study reported for the first time the endophytic fungi from the roots of H. indicus
and their bioactivity. Our present findings highlight the potential of the isolated endophytes to have therapeutic applications.
Pages: 273-280 | 1406 Views 447 Downloads
Shobha M, Bharathi TR, Sampath Kumara KK and Prakash HS. Diversity and biological activities of fungal root endophytes of Hemidesmus indicus (L.) R. Br.. J Pharmacogn Phytochem 2019;8(1):273-280.