Author(s):

Neha Chauhan, Chhaya Singh and Shagun Gupta

Abstract: The study was conducted to investigate the medicinal properties and chemical composition of various solvent extracts of Adhatoda vasica (leaves). The in vitro antibacterial activity was assessed by Agar well diffusion method and radical scavenging activity was measured by FRAP assay. The anticancer potential of leaf extracts of A. vasica on human lung epithelial adenocarcinoma cell line (HCC- 827) were tested by MTT assay. In order to find out the compounds responsible for the medicinal activities, GC-MS analysis was performed. Out of the three extracts subjected to determine the antibacterial activity against the pathogens used in the study, aqueous extract was found to be the best antibacterial agent at the concentration of 0.5mg/100µl. The MIC was tested at various concentrations from 0.5-0.0156 mg/ml for all the plant extracts. MIC/MBC index was also determined and values were less than 4 so all the extracts were bactericidal in nature. The antioxidant powers of all the extracts were assessed and it was concentration dependent. The potency of plant extracts to inhibit the growth of cancerous cell line was recorded in terms of decrease in viable cell count as compared to the control value and it was found to be dose dependent and time dependent. The Phalloidin staining was performed to check the structure of cytoskeleton of control and treated cells. The screening of A. vasica leaves extracts results have shown significant antibacterial, antioxidant and anticancer properties due to the presence of therapeutically important constituents which can be used in the development of herbal formulations in treating respiratory diseases.

Pages: 428-434  |  1146 Views  304 Downloads

Download (626KB)