Author(s):

Noor E Mujjassim, Mallik M, Nenavath Krishna Kumar Rathod and Nitesh SD

Abstract: Heterozygous nature and linkage drag often influence stacking of desirable genes from wild sources in conventional breeding for crop improvement. Recombinant DNA technology and transgenesis have enabled transformation of alien gene into plants across the kingdom barriers. The release of genetically modified (GM) crops into agricultural production has raised considerable debate, especially among the general public, politicians and bureaucrats. To meet this concern, cisgenesis were developed as new tools in crop modification and plant breeding. The cisgenesis concept imply that plants must be transformed with genetic material derived from the species itself or from closely related species capable of sexual hybridization and also foreign sequences such as ‘Selectable marker genes’ and ‘Vector backbone’ sequences should be absent. Intragenic vector construction involves identifying functional equivalents of vector components within plant genomes and using these DNA sequences to assemble vectors for plant transformation.

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