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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 8, Issue 1 (2019)

In vitro studies on the effect of rhizobacteria, Phytotherapeutic substances and chemicals on egg hatching of Meloidogyne graminicola

Author(s):

Vinod Kumar, KK Verma and Anil Kumar

Abstract:
Nematicidal activity of rhizobacteria, Phytotherapeutic substances and chemicals on egg hatching of was assayed against Meloidogyne graminicola. In laboratory assays extracts from Azadirachta indica, Phyllanthus niruri, Achyranthes aspera, Eucalyptus globulus and Xanthium strumarium, free culture filtrate from strains of Pseudomonas fluorescens (P36), Gluconacetobacter diazotrophicus (33-47) and Azotobacter chroococcum (Mac 27,HT 54, HT 57) from chemicals, carbosulfan and cartap hydrochloride showed inhibitory effect on the hatching of eggs of M. graminicola. The maximum hatching inhibition observed in Azotobacter chroococcum HT 54, A. indica and carbosulfan with a 19.3 %, 19.0 %, 14.3 % respectively in 1:5 concentration in all exposure period. Minimum hatching was recorded with A. indica i.e. 8.0 larvae at 1:5 followed by Xanthium strumarium (12.7) at 1:5 after 24 hrs. Among chemicals, carbosulfan showed maximum inhibitory effect followed by cartap hydrochloride. Minimum hatching was recorded with carbosulfan i.e. 14.3 larvae at 1:5 followed by cartap hydrochloride (15.8) at 1:5 after 8 days of exposure. The maximum egg hatching i.e. 94.7 larvae at 8 days was recorded with distilled water check. The rate of hatching was inversely proportional to concentration of substances with of exposure period, as it decreased with increases in concentration viz., 1:5, 1:10, 1:20, 1:40 and 1:80.

Pages: 2593-2597  |  288 Views  34 Downloads

How to cite this article:
Vinod Kumar, KK Verma and Anil Kumar. In vitro studies on the effect of rhizobacteria, Phytotherapeutic substances and chemicals on egg hatching of Meloidogyne graminicola. J Pharmacogn Phytochem 2019;8(1):2593-2597.