Author(s):

Ahamedemujtaba V, Cherian AK, Namitha PM, Louis VIMI and Beena S

Abstract: Cucumber mosaic virus (CMV) causing infectious chlorosis disease of banana was subjected to virus indexing and characterized on basis of biophysical properties. The present investigation results have revealed the occurrence and association of Cucumber mosaic virus with infectious chlorosis disease based on pathogenicity assay, serology (DAC-ELISA) and electron microscopy (EM). The virus developed necrotic localized symptomatic lesions on local lesion host, cowpea (Vigna unguiculata). DAC-ELISA was performed with crude sap, specific antiserum and alkaline phosphatase-linked secondary antibodies (1:10000 dilutions). The mean absorbance at 405 nm for negative and positive samples was 0.1255 and 0.413 respectively, while virus infected samples recorded two times more than the values of negative controls with values ranging between 0.322-0.413. The titre of antisera (1:1000) used in this study gave higher reactivity and it could give clear difference between healthy and infected samples. EM done by leaf dip method showed isometric particles of 28 nm in diameter. Biophysical properties of CMV isolate under investigation for Thermal inactivation point (TIP) is approximately between 65-70°C, the Dilution end point (DEP) ranged between 10-4 to 10-5, longevity in vitro got inactivated from 3rd day onwards at room temperature (28°C) and from 7th day onwards in refrigerated conditions (4°C).

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