Journal of Pharmacognosy and Phytochemistry
Vol. 8, Issue 1 (2019)
Evaluation of genetic diversity in selected Morus genotypes using molecular markers
Author(s):
Suraksha Chanotra, RK Bali, RK Salgotra, K Bali and GK Rai
Abstract:
An attempt was carried out in the present investigation to estimate the extent of genetic diversity and relationship among forty four mulberry genotypes using SSR markers. A set of 26 SSR primers were used to assess the genetic diversity which generated a total of 182 bands with an average of 6.96 alleles per locus. Primer M6 produced maximum of 15 alleles per locus and minimum of 3 alleles per locus in primer Mul3SSR91. Cluster tree grouped 44 genotypes into four main clusters, A, B, C and D with sub-clusters and sub-sub clusters. Highest PIC value was recorded for SSR marker M6 (0.901) and lowest for primer Mul3SSR91 (0.486) with an average of 0.724. Genotype, NS-1 and NS-2 were identified as duplicates while other genotypes showed least similarity and recorded availability of diversity among germplasm.
Pages: 2617-2622 | 917 Views 203 Downloads
Suraksha Chanotra, RK Bali, RK Salgotra, K Bali and GK Rai. Evaluation of genetic diversity in selected Morus genotypes using molecular markers. J Pharmacogn Phytochem 2019;8(1):2617-2622.