Effects of ethanolic leave extract of soursop (Annona muricata L.) on human colorectal cancer line: cell viability and in silico study to cyclin d1 protein

Debby Desmarini; Murdani Abdullah; Puji Sari; Luluk Yunaini; Fadilah Fadilah

Effects of ethanolic leave extract of soursop (Annona muricata L.) on human colorectal cancer line: cell viability and in silico study to cyclin d1 protein

Author(s):

Debby Desmarini, Murdani Abdullah, Puji Sari, Luluk Yunaini and Fadilah Fadilah

Abstract:

Introduction: Colorectal cancer is a pathological transformation of normal colon and rectum epithelial that becomes an abnormal tissue mass, due to the overexpression of cyclin D1 protein that inducing the high proliferation of colorectal cell. Treatment and prevention of colorectal cancer could be done naturally by consuming leave extract of Annona muricata L. (soursop). Soursop is known for many phytochemical components such as alkaloid, annonaceous acetogenin, phenol, and flavonol that serve as an anti-cancer.

Method: The research was used HT-29 colorectal cell that given ethanolic leave extract of soursop with 278 μg/mL concentration, 5-Fluorourasil (5-FU) with 88 μg/mL concentration as positive control, solvent control, dan cells control as negative control. The parameters are cell viability with MTT Assay and analysis of molecular docking from ethanolic leave extract of soursop to cyclin D1 protein with molecular operating environment (MOE) 2013.08 software.

Result: Percentage of viable HT-29 cell line decrease in accordance with increasing concentration and the lowest percentage of viable cell is 2 x cytotoxicity concentration 50 (CC50) after ethanolic leave extract of soursop treatment (40,4±1,3%) was compared to 5-FU (30,68±0,93%), solvent control (97,2±1,4%), and cells control (100%). Analysis of molecular docking to cyclin D1 protein was obtained N-hexadecanoic acid and phytol molecules that have the lowest free energy (ΔG), i.e 9,7755 kkal/mol and -7,2147 kkal/mol.

Conclusion: Ethanolic leave extract of soursop causes decreasing cell viability of HT-29 cell line on 2 x CC50 concentration was compared to 5-FU, solvent control, dan cells control. N-hexadecanoic acid and phytol molecules have ability to inhibit cyclin D1 protein.

Pages: 232-237 | 867 Views 278 Downloads

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Journal of Pharmacognosy and Phytochemistry

How to cite this article:

Debby Desmarini, Murdani Abdullah, Puji Sari, Luluk Yunaini and Fadilah Fadilah. Effects of ethanolic leave extract of soursop (Annona muricata L.) on human colorectal cancer line: cell viability and in silico study to cyclin d1 protein. J Pharmacogn Phytochem 2019;8(2):232-237.

Pharmacognosy

Journal of Pharmacognosy and Phytochemistry

Vol. 8, Issue 2 (2019)

Effects of ethanolic leave extract of soursop (Annona muricata L.) on human colorectal cancer line: cell viability and in silico study to cyclin d1 protein

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