Author(s):

Perumalla Srikanth, Ann Maxton and Sam A Masih

Abstract: The cultivation of transgenic cotton is increased in a large scale since from its introduction globally. Bacillus thuringiensis is produced crystalline proteins, these proteins were used in transgenic cotton to control insect pest. Resistance in insects is a major problem in transgenic cotton growing regions in all over the world especially, pink boll worm developed resistance in all major cotton regions in India. It was noticed in our studies that the expression levels of Cry1Ac in different plant parts such as upper leaves, lower leaves, sepals and boll bracts was increased while increasing refuge percentage. Planting refuge with alternatively with Bt cotton also increase the Cry1Ac expression levels compare with border refuge. An optimized unique combination of refuge crop viz., 75% Bt with 25% nBt showed higher expression level of Cry1Ac and highest yield was also achieved. This combination is useful to counter insect resistance and the staking of two toxin Cry proteins in same transgenic cotton.

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