Author(s):

Nandha AK, Mehta DR, Tulsani NJ, Umretiya N, Delvadiya N and Kachhadiya HJ

Abstract: Common bread wheat is one of the most crucial cereal crops for human nutrition. Due to its complex structure, the study of molecular biology of this plant become important for further improvement which can provide better quality and quantity of final yield. Wheat is highly sensitive toward the temperature variation as little change in temperature lead to high loss. High quality transcriptome analysis can be helpful to understand this complexity and allow us to do desirable changes. For study two genotypes have been used, one was heat susceptible (WH 147) and second was heat tolerant (GW 451). The transcriptome analysis of wheat was done using IonS5 which gives 287,080 and 263,340 numbers of transcripts in GW 451 and WH 147, respectively. These data were used for preparation of further unigene assembly. Determination of various proteins was done using four different databases and retrieved proteins were matched with different species to get top hits. Gene ontology of stressed and control plant was categorised in three categories and 49 subcategories which showed versatility in each category. The functional analysis also gives functional categories between two genotypes during control and stressed condition. Differential gene expression analysis indicated the notable variation in various genes under stressed condition in both genotypes, which were identified by their up regulated or down regulate level. Identification of pathways which gives the buffering ability to plant under heat stress condition were identified using Kyoto Encyclopedia of Genes and Genomes pathway analysis. Different types of heat shock proteins were identified in this study among which high molecular weight HSP and moderate molecular weight HSP were abundance then low molecular weight. This data can be useful for the further characterisation of molecular analysis of wheat which can helpful to generated heat tolerance variety.

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