In the present study, the phytochemical analysis determines Litsea laevigata
leaf and stem extract used as non-polar to a polar solvent of pet-ether, chloroform, acetone methanolic and water. Leaf and stem investigated by applying established in vitro
studies. L. laevigata belongs to the Lauraceae family.
Method: To confirm the fingerprint outline of Litsea laevigata using high-performance thin layer chromatography (HPTLC) procedure. Methods: Preliminary phytochemical screening was performed and HPTLC investigations were provided an explanation. CAMAG HPTLC system provided with Linomat V applicator, TLC scanner 3, Reprostar 3 and WIN CATS-4 software was done.
Results: Preliminary phytochemical screening of the extract showed the presence of flavonoid, reducing suger, tannins, saponnins, phenolic compounds, steroid and terpenoid.
HPTLC finger printing of methanolic extract of leaf and stem revealed 3 and 9 peaks of phenol compound delectation, with Rf values start from 0.02 to 0.67 in leaf and 0.03 to 0.90 in stem. Flavonoid delectation of leaf and stem revealed 6 and 7 peaks with Rf values start from 0.02 to 0.99 in leaf and 0.05 to 0.99 stem.
Conclusions: It can be assumed of HPTLC fingerprint investigation of leaf and stem extract of Litsea laevigata can be done as a characteristic tool for the accurate identification of the plant and it is valuable as a phytochemical marker and also an immeasurable estimator of transmitted variability in plant communities.