Author(s):

P Anusha and S Rajkumar Immanuel

Abstract: Hygrophila auriculata is one of the important medicinal plants found in India, which is Acanthaceae family and is distributed in tropical and subtropical region of India. The plant is used in cancer and tubercular fistula. Root and seeds used as tonic for asthama and dysentery. The leaves, roots and seeds of this plant are traditionally used for the treatment of inflammation, jaundice, hepatic obstruction and urinary infection. The aim of the present study was to evaluate the antioxidant and antibacterial activities of methanol leaves extract of H. auriculata. Antioxidant assays such as DPPH^· radical, superoxide (O₂^·-) radical, ABTS^·+ radical cation, Fe³⁺ reducing power and phosphomolybdenum reduction assays were carried out for evaluating antioxidant activities. The maximum DPPH^· radical scavenging activity of methanol leaves extract was 72.82±0.19% at 120 µg/mL concentration and the IC₅₀ was 83.88 µg/mL concentration. The maximum superoxide radical scavenging activity of methanol leaves extract was 81.72±0.28% at 120 µg/mL concentration and the IC₅₀ was 20.60 µg/mL concentration. The maximum ABTS^●+ radical cation scavenging activity of methanol leaves extract was 88.93±0.23% at 30 µg/mL concentration and the IC₅₀ was 11.75 µg/mL concentration. The maximum of Mo⁶⁺ reduction and Fe³⁺ reduction of methanol leaves extract were 79.68±0.41% and 47.88±0.47% at 120 µg/mL concentrations and the RC₅₀ were 56.35 µg/mL and 125.31 µg/mL concentrations respectively. The antibacterial activity was carried out by well diffusion method and showed maximum zone of inhibition of 16 mm for Proteus vulgaris at 500 µg/mL concentration.

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