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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 8, Issue 3 (2019)

Polyacrylamide gel electrophoresis and silver staining for detection of rotavirus in goat fecal samples

Author(s):

Vandana Gupta, Megha Pandey, Anju Nayak, Shweta Rajoriya and Smita Bordoloi

Abstract:
The present study was undertaken to detect the presence of Rotavirus in non-diarrhoeic goat fecal sample by RNA-PAGE and Silver staining. Around 70 fecal samples from non-diarrhoeic goats aging between 2 months to 2 year were subjected to RNA- PAGE after nucleic acid extraction, of which none of the sample was found to show 11 segments migration pattern typical of Rotavirus. RNA-PAGE serves as a simple and inexpensive technique, for detection of rotaviral double-stranded RNA genome in faecal specimens by PAGE in combination with silver staining.

Pages: 472-475  |  1119 Views  297 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Vandana Gupta, Megha Pandey, Anju Nayak, Shweta Rajoriya and Smita Bordoloi. Polyacrylamide gel electrophoresis and silver staining for detection of rotavirus in goat fecal samples. J Pharmacogn Phytochem 2019;8(3):472-475.

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