Author(s):

Madhuri B Gawande, SJ Gahukar, Aamrpali Aakhare, DR Rathod, Yashoda Etther and RL Bhakre

Abstract: “Molecular characterization of F₂ segregating population for fibre properties of cotton” was attempted using phenotypically diverse parentages from Dr. PDKV, Akola and their molecular characterization for fibre quality parameters using simple sequence repeat (SSR) markers. Two F₂ populations were developed [cross-I (PKV-Rajat x MCU-5) and cross-II (AKH-081 x MCU-5). Staple length (SL) and staple strength (SR/Sst) were recorded on selected 40 F₂ populations in both the crosses. Polymorphism were observed for SL and SR/Sst in both the populations. In case of molecular markers studied a total of 64 SSR markers found good polymorphic between the parents on 26 chromosomes out of 116. The polymorphism percent varies from chromosome to chromosome. Highest was found on chromosome 22, 23 and 24 (100%), whereas lowest was obtained on chromosome 13 and 20 (25%). The range of polymorphism was 25 to 100%. Tri-nucleotide repeats of microsatellite markers found polymorphic than di and polynucleotide. 12 SSR markers were consistently linked with different traits studied. Three SSR NAU769, BNL2656 and BNL226 linked with number of staple length/plant on chromosome 3, with LOD 2.61-2.62, PVE 0.15-19.52%. Two SSR BNL2920 and BNL3920 were linked with staple strength on chromosome 15, with LOD 7.54-3.00, PVE 0.69-6.18%.x

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