Abstract:
Objective: Use of Myrobalan to heal Human ailments is practiced since Ayurveda, Unani and Siddha Medicines. This has resourced evaluating medicinal properties of species of Genus:
Terminalia L., globally. Focusing this, Australian Almond-
Terminalia muelleri Benth. was Bioprospected for Antioxidant potential by 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) & Nitric oxide (NO) Free Radical Scavenging Assay.
Method: Aqueous ethanolic bark extract (AEBE) of T. muelleri (Tmu AEBE) was Soxhlet extracted and condensed using Rotovap. Different weighing concentration (5-50 µg/mL) of Tmu AEBE were experimented for antioxidant potential, against synthetic antioxidant- Ascorbic acid. DPPH Free Radical Scavenging Activity (FRSA) was experimented by Blois method while NO FRSA was experimented by Nims et al. method. Half maximum effective concentration (EC50) and its inverse called as Antiradical power (ARP) was also calculated for the first time for T. muelleri.
Results: FRSA of Tmu AEBE obeyed Concentration dependent expression following Non Linear progression. These DPPH & NO FRSA results were expressed as % Free Radical Scavenged (%). NO FRSA results were expressed as Mean ± S. D. of three replicates (n=3) and analyzed statistically using One way ANNOVA. Quantal concentration dependent Radical Scavenging curves were processed for calculating EC50 (expressed as µg/mL) using Origin Pro 8.5.
Conclusion: Phytoconstituents constituting Tmu AEBE constituted to DPPH & NO Free Radical Scavenging Potential, in-vitro. Thus these phytoconstituents will be structured using modern analytical instrumentations and their Antioxidant capacities will be Bioprospected, individually.