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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 8, Issue 3 (2019)

Transcriptome sequencing, De novo assembly from three developmental growth stages in fenugreek (Trigonella foenum-graecum L.)

Author(s):

Shubha R and Rukam S Tomar

Abstract:
Fenugreek (Trigonella foenum-graecum L.) popularly known as “Methi” belongs to family fabaceae and subfamily papilionaceae. Gujarat Methi Variety 2 was selected for transcritome study from the three developmental stages that were vegetative stage (20-30 DAS), reproductive stage (50-60 DAS) and maturity stage (80-90 DAS) which were sequenced using Ion S5 genome sequencing machine. The de novo assembly yielded assembled reads from vegetative stage, total reads were 59,306, and the largest contig was 5773 bp and total number of base pairs were 2,562,931,387, with a consensus length of 39 bp. Similarly, assembly yielded assembled reads of 30,648,677 from reproductive stage with a consensus length of 40 bp, total number of contigs generated in reproductive stage, were 57,415 and the largest contig was 7,167 bp, total number of base pairs were 3,143,890,944. In maturity stage assembled reads of 16,924,096 with a consensus length of 81 bp were generated. Total number of contigs generated in maturity stage were, 46,721 and largest contig was 4,434 bp and the total number of base pairs were 3,205,640,970.

Pages: 1779-1794  |  1065 Views  427 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Shubha R and Rukam S Tomar. Transcriptome sequencing, De novo assembly from three developmental growth stages in fenugreek (Trigonella foenum-graecum L.). J Pharmacogn Phytochem 2019;8(3):1779-1794.

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