The present paper demonstrates the effect of different media and combinations of growth regulators to standardize different stages of in vitro propagation protocol for mass production of pomegranate cv. Super Bhagwa. In this investigation, shoot tips were used as explant and explant necrosis, culture contamination and culture establishment were found to be 7.61%, 16.39% and 76.66%, respectively. The combination of α-Naphthaleneacetic acid (NAA) and 6-Benzylaminopurine (BAP) was more responsive for culture establishment. T6 medium (Modified MS + Adenine Sulphate (60mg/l) + L-Arginine (60mg/l) + 1mg/l Kinetin + 0.1mg/l TDZ + 0.2 mg/l NAA + Undisclosed media supplements) registered maximum proliferation rate (3.800), however, MS medium supplemented BAP + very low concentration of TDZ/ZR and adenine sulphate (60 mg/l) along with 0.1/0.2 mg per litre NAA produced tallest shoot and high average shoot length.

In vitro rooting of proliferated shoots on Woody Plant medium (Lloyd and McCown, 1980) (WPM medium), irrespective of growth regulator concentration and type, took lesser number of days for root initiation as compared to half strength MS medium (Murashige and Skoog, 1962). Major roots per shoot (5.00) and the rooting percent (76%) were found highest in WPM medium supplemented with 1.0 mg/l NAA as compared to other treatments. Use of half strength concentration of MS medium and full strength WPM was an attempt to reduce the salt concentration to enhance rooting in vitro.

Arbuscular Mycorrhizal Fungi (AMF; predominantly Glomus intraradices) inoculated plants depicted higher relative leaf water (85.27%) and more leaf chlorophyll content (2.17) as compared to non-inoculated micropropagated pomegranate plants of cv. Super Bhagwa.