Author(s):

Sritoma Banerjee and Kalyan Kumar Sen

Abstract: In this study, a hydroalcoholic solvent system was used to prepare Chelidonium majus L extract. Prepared extract was undergone qualitative and quantitative evaluations. Thin layer chromatography was done by applying different solvent systems to acquire knowledge on separation of components. For further analysis related to the plant extract, a suitable UV spectrophotometric method was developed and validated. With due diligence of WHO and ICH guideline Q2(R1) on validation of analytical procedure, cardinal and prime attributes like linearity, range, precision, accuracy, system suitability, robustness, solution stability were analysed. Qualitative evaluations suggested prepared extract contain alkaloid, flavonoid, phenolics, carbohydrates, proteins, saponin, phytosterol etc. Separation process of pure component needed further in depth analysis. Developed UV method was suitable, accurate, precise and robust with the change in laboratory and instrument.

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