Author(s):

Jaya Rathore, AK Singh and Dr. S. V. S. Chauhan

Abstract: The present investigation was carried out in three seasons during summer-2016 to Kharif-2017 at Research Farm, College of Agriculture, Gwalior. On the basis of per se performance four genotypes were selected by screening sixteen genotypes which were collected from two region (ARS, Durgapura, Jaipur & Dept of Agronomy, COA, RVSKVV, Gwalior). Four selected genotypes were irradiated by using 10 doses of physical mutagen (gamma rays). The irradiated seeds along with controls were sown to raise M1 generation without replication. Progeny of individual selected mutant plants from M₁ generation were grown using compact family block design with two replications. Mutant lines viz., 42-10-RGC-1038 followed by 133-35-RGC-1038, 133-35kR-RGC-1038,144-40kR-RGC-1038, 139-40kR-RGC-1038, 48-10kR-RGC-1038, 140-40kR-RGC-1038, 141-40kR-RGC-1038, 166-50kR-GST-15-204, and 74-15kR-GST-15-204 were identified for high gum content than best control RGC-1066 (26.85) and these mutant lines were also recorded for high yield. High Proline content was recorded in mutant line viz., 161-50-GST-15-204 (199.50) followed by 159-50kR-RGC-1066, 165-50kR-GST-15- 204, 153-45kR-GST-15-204, 154-45kR-GST-15-204, 130-35kR-RGC-1038, 128-35kR-RGC-1038132-35kR-RGC-1038 and these mutant lines was also found for high yield. Thus, we can say that mutant lines which show high proline content can survive in stress condition and can give high yield. Proline content was found lowest at 5 kR and highest at 50kR. High gum content was also found at higher dose (40kR) over the control. Thus, by this result it can say that higher dose can beneficial to increase proline content and gum content. Recently novel hydrogels were synthesized from guar gum. Hydrogels helps combating problem of water scarcity in arid regions by being super absorbent (ie. Nearly it can absorb 600ml water/gm of hydrogel) and are being evaluated as soil conditions. At last we can concluded that Mutant lines which has high proline content and high gum content can boon for Arid and semi-arid zones.

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