Author(s):

Bhavna Dwivedi, Sherendra Kumar Sahu and Skand Kumar Mishra

Abstract: Antibacterial property of different medicinal plants are well established and now- a- days they are using as an alternative medicine. In this experiment, the methanolic and aqueous leaf extract of Withania somnifera, commonly known as ashwagandha were evaluated for antibacterial activity against gram positive Staphylococcus aureus. High performance thin layer chromatography (HPTLC) based purified and characterized extracts were subjected to analysis disk diffusion inhibitory test and oxidative stress marker-reactive oxygen species (ROS) generation in bacteria. Results obtained indicated that extracts contained significant amount of phenolic, flavonoids and other phytoconstituents like alkaloid, tannin, terpenoids, steroids. However, the calculated minimum inhibitory concentration of extract was 100±7.5 µg. Further, a significant increased of ROS level was observed in treated bacteria concentration range from 20-800 µg/ml respectively (835±65, 1500.75±126.55, 2000±250.65, 4600±137.55, 4100±830.75, 4500±1200.59 with respect to control). Thus, the results suggested that extracts contained phenolic and flavonoid compounds which could be responsible for oxidative stress and bacterial death.

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