Author(s):

Kosasih Kosasih, Wahono Sumaryono, Agus Supriono and Diky Mudhakir

Abstract: Cantigi (Vaccinium varingiaefolium (Blume) Miq.) is endemic plant of Java dominating sub-alpine area of Mount Tangkuban Parahu. Its young leaves are red and will turn green. Previous study reported that ethyl acetate extract of young leaves had antioxidant activity using DPPH method and cytotoxic activity against leukemia L1210 cell lines. This experiment was designed to know whether ethyl acetate (EA) extract of Cantigi leaves had cytotoxic activity against Artemia salina L. larvae, breast cancer cell lines T47D and MCF-7, and normal cell line Vero. Dried leaves were extracted with maceration method, firstly, using hexane, and then EA. Obtained EA extract was dried using rotary evaporator. EA extract was examined its cytotoxic activity using Brine Shrimp Lethality Test (BSLT) method, against breast cancer cell lines T47D and MCF-7, and normal cell line Vero using Microculture Tetrazolium Salt (MTT) method. As the positive control, cisplatin was used. This experiment showed that LC₅₀ of the extract was 320.83 ppm, IC₅₀ against breast cancer cell line T47D was 75.23 ppm, IC₅₀ against breast cancer cell line MCF-7 was 88, 89 ppm, IC₅₀ against normal cell line Vero was 305 ppm, and IC₅₀ of cisplatin against breast cancer cell line T47D was 3.91 ppm. It can be concluded that the EA extract has cytotoxic activity with LC₅₀ < 1000 ppm, strong cytotoxic activity against both breast cancer cell lines tested with IC₅₀ < 100 ppm, has weak cytotoxic activity against normal cell line Vero with IC₅₀ > 300 ppm, and potentially as a candidate for breast cancer therapy.

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