Author(s):

Muhammad Rafi Bawari and T Narendrappa

Abstract: Bacterial wilt caused by Ralstonia solanacearum is a lethal disease of brinjal in tropical, subtropical and warm temperate regions of the world. In present investigation, isolation of the biochemical and physiological characterization of R. solanacearum was conducted. The results of the various cultural and biochemical characters revealed that the bacterium isolates were rod shaped, Gram negative, non-capsulated. They appeared dull white with light pink colored center on TZC medium and the colonies were highly fluidal producing copious slime. The bacterium was positive for nitrate reduction test and negative for starch hydrolysis and gelatin liquification. Physiological studies viz., Effect of temperature and effect of pH on the growth of R. solanacearum on CPG agar medium was studied. The temperature of 30 ^oC was found optimum for the growth of pathogen as the highest population (196.66 x 105cfu/ml) were recorded at this temperature level followed by 25 ^oC (114.66 x 105cfu/ml) and 35 ^oC (103.33 x 105cfu/ml) and the maximum number of bacterial colonies were recorded at pH 7 (154.00 x 105cfu/ml).

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