Author(s):

Pasupathi E, Murugan M, Harish S and Chinnaiah C

Abstract: Field screening of 88 okra ge­rmplasm was done for resistance against the sweet potato whitefly, B. tabaci and the begomoviruses, okra enation leaf curl virus (OELCV) and bhendi yellow vein mosaic virus (BYVMV) during two seasons (March, June sowing) of 2018 at Attur, Salem Distrct, Tamil Nadu. The lowest mean population of whiteflies was recorded in the okra accessions viz.,Upl mona 2 (0.35), Co 1 (0.4), Abelomoschus moschattus (0.65), Sona (0.78), where as accessions AE 66, IC 113920 and IC 282274 recorded the highest number of whiteflies with a mean population of 3.94, 3.45 and 3.24 adults per leaf. Among the accessions, one wild accession, A. moschattus and one cultivated accession Upl mona 2 did not show any signs of OELCV and BYVMV infection throughout the crop period and exhibited immune reaction (0%) and Upl mona 2 recorded the maximum yield. The highest OELCV per cent disease incidence (PDI) was recorded on AE 66 (100) followed by AE 64 (80) and AE 65 (80), while the PDI recorded susceptible check was 100%. The OELCV infected young leaves of selected okra accessions were collected from the screening field and was analyzed using DNA marker specific to coat protein based primer in polymerase chain reaction (PCR). The PCR amplified fragments were sequenced and compared for OELCV, and showed 99% similar sequence homology of already reported in NCBI data base. The finding could be highly useful in okra breeding programs against OELCV.

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