Author(s):

R Yogeswari, A Arivuchelvan, S Murugasen, GA Balasubramaniam, P Selvaraj, A Jagadeeswaran and A Raja

Abstract: Lead is a well known non-biodegradable toxic heavy metal in the environment and now, it has become a global issue. Lead is a cumulative toxicant that affects multiple body systems. Garlic, Allium sativum L. is a member of the Alliaceae family, has been widely recognized as a valuable spice and a popular remedy for various ailments. Hence in this study the effect of lead acetate on zebrafish embryo/larvae and amelioration by garlic aqueous extract was assessed. Normally dividing 5 hpf (hours post fertilization) embryos were allotted into different groups, in six well plates. Two control groups one with plain embryo water and the other with garlic aqueous extract. Lead acetate was exposed at three dose levels 0.1, 0.5 and 1 ppm to next three groups. The other three groups were exposed with lead acetate at three levels along with 1 µg garlic aqueous extract. To evaluate the livability, embryos and larvae were counted at 5, 24, 48, 72, 96 hpf and 5, 6, 7 and 8 dpf (days post fertilization). From 24 hpf to 8dpf control and drug control groups differ significantly (P≤ 0.01) from all other groups. At all hours and days post fertilization, the livability of the embryo/larvae was affected dose dependently in the lead acetate treated groups and as well the garlic aqueous extract has protective effect, in dose dependant manner.

Pages: 189-193  |  1417 Views  412 Downloads

Download (764KB)