Objectives: The present study was to isolate, characterize the secondary metabolites from the Diospyros malabarica (Desr.) Kostel stem bark and to evaluate their in-vitro antidiabetic activities.

Material and Methods: The D. malabarica stem bark powder was subjected to extraction, fractionation and column chromatography to isolate compounds. The identical eluents were collected and monitored on TLC and characterized them by IR, Mass and 1HNMR and 13CNMR spectra. Later, they were subjected to in-vitro antidiabetic studies such as α-amylase, sucrase and α-glucosidase inhibitory activities.

Results: Four known compounds – betulin, betulinic acid, 16-hydroxy betulinic acid and lupeol were isolated from chloroform fraction of D. malabarica stem bark. The spectroscopic data confirmed their structures from reported compounds. The isolated compounds betulin and betulinic acid exhibited α-amylase and α-glucosidase inhibitory activities.

Conclusion: We isolated four known compounds from chloroform fraction of D. malabarica stem bark and betulin and betulinic acid exhibited significant in-vitro antidiabetc activities.