Pooja Sahu

A study entitled “Inheritance and molecular mapping of fertility-restoring (rf) gene for WA cytoplasmic male sterility system in rice using SSR markers” was conducted at Research cum Instructional Farm and Plant Molecular Biology Laboratory, IGKV, Raipur, Chhattisgarh considering three CMS lines viz., IR 58025A, CRMS 32A and IR 79156A and eleven testers viz., IR73007-44-1-2-3, ET-1-10, R1120-102-3-88-1, ET-1-12, IR-71606-1-1-4-2-3-1-2, R1138-696-821-3-1, R1521-950-6-843-1, R1529-1183-1-1041-1, R1530-1196-1063-1, PKV-HMT and SAMPADA. Thirty three F1s were generated in line x tester pattern, based on pollen and spikelet fertility, seven cross combinations were identified as potential restorers. Inheritance study in F₂ population consisting five populations possessing WA cytoplasm revealed that, two populations viz. IR58025A/IR-73007-44-1-2-3 and IR58025A /ET-1-10 were identified to be governed with dominant monogenic gene action (3:1) for fertility restoration. Whereas, IR58025A/R-1120-102-3-88-1 showed a segregation ratio of 12:3:1 indicating two major genes with dominant epistasis involved in fertility restoration and IR58025A/ET-1-12 indicating two major genes with recessive epistasis involved in fertility restoration with digenic ratio 9:3:4 for fertility restoration. Cross combination IR58025A/IR-71606-1-1-4-2-3-1-2 does not show any type of interaction. Molecular analysis conducted in F₃ generation consisting of two crosses viz., IR 58025A/IR-73007-44-1-2-3 and IR 58025A/ET-1-10 indicated that, out of 10 SSR markers , 3 markers viz., RM3873, RM258 and RM3233 has been found polymorphic within the population of IR58025A /ET-1-10 cross combination. But none of the markers segregate according to the population. Hence, no linkage has been detected in the population with the markers. The present study is useful in planning a sound breeding strategy for development of superior restorer in a hybrid breeding program.

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