Journal of Pharmacognosy and Phytochemistry
Vol. 9, Issue 1 (2020)
Influence of media, pH and temperature on the growth of Sclerotium rolfsii (Sacc.) causing collar rot of chickpea
Author(s):
Bana Sravani and Ram Chandra
Abstract:
Sclerotium rolfsii is the most devastating pathogen causing collar rot of chickpea especially in tropical and sub-tropical countries where high temperatures prevail during monsoons. An attempt was made to study the best suitable medium and physiological factors such as temperature and pH on the growth of pathogen. Among the six different media tested, Potato Dextrose Agar (PDA) and Yeast Potato Dextrose Agar (YPDA) medium were found to be highly supportive for the pathogen growth resulting full growth (9cm) within 7 days and least growth was observed with Paddy Husk medium (6.33cm). Among different pH studied, maximum growth was reported at pH 6 followed by 7, 8, 5 and 8.Fungal growth was found to be vigorous at 30 ℃ temperature where radial growth was observed to be maximum (9cm) within 5DAI followed by 25 ℃, 20 ℃, 15 ℃ and least growth at 35 ℃.
Pages: 174-178 | 1916 Views 740 Downloads
Bana Sravani and Ram Chandra. Influence of media, pH and temperature on the growth of Sclerotium rolfsii (Sacc.) causing collar rot of chickpea. J Pharmacogn Phytochem 2020;9(1):174-178.