Author(s):

Thoudam Bhaigybati, Suchitra Sanasam, Jessia Gurumayum, GC Bag, Loitongbam Ranjit Singh and Pukrambum Grihanjali Devi

Abstract: The present study was carried out to analyze the phytochemical constituents, antioxidant activity, total phenolic content, total flavonoid content and antimicrobial activity of Ipomoea aquatica Forsk. Preliminary phytochemical analysis was done using standard procedure. Total phenolic content, total flavonoid content and antioxidant activities were determined spectrophotometric ally in crude aqueous and methanolic extracts. The in vitro antimicrobial activity was determined by well diffusion method against four MTCC bacterial species and three MTCC fungal species. Preliminary phytochemical analysis indicated the presence of most of the phytochemicals except steroid and phlobatannins. Antioxidant activities increased with increase in concentration of the extract. The methanolic extract showed a better result than the aqueous extract. Clear antimicrobial activity was observed against Staphylococcus aureus and Escherichia coli using methanolic extract while aqueous extract showed moderate activity. Moderate activity was noted against Pseudomonas aeruginosa using methanolic extract whereas the aqueous extract was found to be inactive towards the same organism. Antifungal activity species was absent. GC-MS analysis was carried out to identify the bioactive compounds in methanolic extract as methanolic extract was showing a better result. The present study demonstrates the antioxidant and antimicrobial activity of Ipomoea aquatica Forsk due to the various phytochemicals present in the extract which confer their traditional uses as food and medicine.

Pages: 2335-2342  |  2672 Views  1487 Downloads

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