Journal of Pharmacognosy and Phytochemistry
Vol. 9, Issue 2 (2020)
Fractional Extraction, isolation and identification of biologically active compounds from Melaleuca leucadendron L. to control the vectors of dengue and filariasis
Anju Viswan K, Pushpalatha E, Arivudainambi S and Selvamuthukumaran T
To find out the larvicidal activities of Melaleuca leucadendron
(L.) against Culex quinqueafsciatus Say and Aedes albopictus (Skuse), and to isolate the active compounds present in the most active fraction of the plant extracts.
Cold extracts of M. leaucadendron leaves were taken using methanol as solvent and fractionation was done using the solvents n-hexane, ethyl acetate and acetone by column chromatographic method. Bioassays were conducted using all these extracts and LC50 were calculated. The structural elucidation of the compounds of most active fraction isolated from chromatographic studies after bioassay was done by LCMS and NMR spectroscopy.
M. leucadendron treated larvae shown restless activity in Ae. albopictus more than in Cx. quinquefasciatus. The activity of column fractions of M. leucadendron was in the order Chloroform > Hexane (H) >: Ethyl acetate (EA). The compounds present in the most active fraction was citral and α- gurjunene.
Conclusion: From the results it has been proved that the plant is having potential larvicidal activity.
Pages: 68-73 | 1043 Views 341 Downloads
Anju Viswan K, Pushpalatha E, Arivudainambi S and Selvamuthukumaran T. Fractional Extraction, isolation and identification of biologically active compounds from Melaleuca leucadendron L. to control the vectors of dengue and filariasis. J Pharmacogn Phytochem 2020;9(2):68-73.