1. Procurement of Raw Material: Collection of part of plant in which alkaloids are present in great concentration like roots, flowers, seeds, barks, fruits etc.

2.. Extraction and Fractionation: Extraction can be done by using different techniques like Percolation and Soxhlet Extraction and Fractionation by using Column Chromatography.

3. Isolation and Characterization

4. These isolation and Characterization can be done by using TLC and Column Chromatography.

5. Standardization

1. Authentication (Stage of collection, Parts of the plant collected, Regional status, Botanical identity like Phytomorphology microscopically and Histological analysis, Taxonomical identity, etc.)

2. Chromatographic and spectroscopic evaluation. TLC, HPTLC, HPLC methods will provide qualitative and semi quantitative information about the main active constituents present in the crude drug as chemical markers in the TLC fingerprint evaluation of herbals (FEH). The quality of the drug can also be assessed on the basis of the chromatographic fingerprint.

3. Chromatographic analysis of herbals can be done using TLC, HPLC, HPTLC and GC, UV, Fluorimetry, GC-MS, etc.