Author(s):

Priya Singh, Inkresh Kumar Verma and Manju Sharma

Abstract: The isolates of Trichoderma obtained from various regions of Pantnagar and Dehradun were screened for their ability to enhance germination and other growth parameters in soybean under in vitro as well as under in vivo conditions. All the fifteen isolates (PT-1, PT-2, PT-3, PT-4, PT-5, PT-6, PT-7, PT-8, PT-9, PT-10, DDNT-1, DDNT-2, DDNT-3, DDNT-4 and DDNT-5) were mass multiplied on the sorghum grains and grounded into fine powder. The soybean seeds were treated with the powdered Trichoderma formulation @ 10g/kg seed and kept in paper towel and incubated at 28±1°C for 7 days under in vitro condition. For in vivo screening, the Trichoderma formulation were applied to the soil @ 10g/ 2kg pot soil, seed treated with Trichoderma powder @ 10g/kg seed and foliar spray of Trichoderma powder @10g/l of water at 15 days after sowing was done. Under in vitro condition the isolate PT-2 (15.50 cm) recorded maximum plumule length while DDNT-4 (15.50 cm) showed highest radical length. However, under in vivo condition DDNT-4 (34.50 cm) recorded maximum shoot length and PT-5 (19.00 cm) showed maximum root length.

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