Author(s):

Mustafid Rasyiid and Ratna Susandarini

Abstract: Honey produced by the giant honeybee (Apis dorsata binghami) is considered as highly nutritious natural product and has long been used as health supplement. This type of honey is collected traditionally by honey hunters using crude method from forest. This study aimed at determining the diversity of pollen and secondary metabolite compounds in honey samples produced by giant honeybee collected from four regencies in Central Sulawesi. Honey samples were collected from Donggala, Banggai, Morowali, and Parigi Moutong. Modified acetolysis method was used for preparing pollen microscopic slides, while TLC method was performed for the detection of secondary metabolite compounds. The results showed that honey samples varied in their pollen diversity, with the number of pollen types found in honey sample from Donggala was 33, Banggai was 36, Morowali was 30, and Parigi Moutong was 57. All honey samples contained five classes of secondary metabolite compounds namely alkaloid, phenolic, flavonoid, tannin, and terpenoid. From this melissopalynological research, it could be concluded that honey samples produced by giant honeybee from Central Sulawesi are of good quality based on their pollen diversity and the content of secondary metabolite compounds.

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