To fulfill the increasing demand for dyes and pigments by the textile industries syntactic dyes comes into existence, which caused a serious environmental problem. Natural dyes are one such alternative to this problem. In this regard the present study was conducted to optimize extraction protocol (by using Box-Behnken Design) and chemical characterization of dye from the Lantana camara. The optimized point for extraction of flower and leaf dye was achieved at 920C, 174 minutes with Mass to liquor ratio MLR 2:100, yielded 29.70% dye and 790C, 36 minutes with MLR 3:100 yielded 26.2% dye respectively. The Methanol and Ethanol extract of flower and leaf dye was subjected to phytochemical screening by using GC-MS and dye extract showed that the presence of n-Hexadecanoic acid and 1,2-Benzenedicarboxylic acid, bis(2-ethylhexyl) in higher concentration. Most of the chemicals found in the dye extract have antibacterial, antifungal and Anti‐Inflammatory properties. Hence, the use of the Lantana camara leaf and flower will reduce the pollution and helps in the management of this invasive weed in the Indian forests.