Author(s):

B Boblina, SK Beura, AG Panda and MK Mishra

Abstract: The efficient management of foliar as well as soil borne pathogens makes Trichoderma one of the most used biocontrol agents in the world. Development of formulations with enhanced shelf life and broad-spectrum activity could pave the way of rapid commercialization of these beneficial microorganisms. Two native isolates of Trichoderma species (Tr Isolate 2 and Tr Isolate 5) were collected from paddy and groundnut rhizospheres respectively. Their vigorous growth in different culture media and profound inhibitory effects on two potential soil borne pathogens namely Rhizoctonia solani and Sclerotium rolfsii as observed in dual culture experiment were the basis of selecting these two isolates among all the seven other isolates collected from different crop rhizospheres for mass production. Present study deals with evaluation of nine liquid substrates and one talc-based formulation. Colony forming units were counted following the serial dilution technique and a gradual reduction was observed over a period of six months. Among various liquid substrates put into test, Coconut water was observed to be the best for both of the isolates (Tr Isolate 2 and Tr Isolate 5) recording the maximum cfu count 170.00 x 10⁷ cfu/ml and 99.00 x 10⁷ cfu/ml of substrate in the first month respectively. So far as talc-based formulations are concerned, the maximum count 44.33 x 10⁷ cfu/g was recorded in the first month in Tr Isolate 2. Similarly, in case of Tr Isolate 5 the highest cfu count of 48.00 x 10⁷ cfu/g was recorded in first month after inoculation.

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