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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 9, Issue 4 (2020)

Development of in-vitro protocol for direct regeneration from thalamus ex-plant of Tagetes patula L. var. Pusa Deep

Author(s):

Soudamani Karjee, Namita, Kanwar P Singh and Sapna Panwar

Abstract:
The effect of growth hormones such as BAP, IAA and KINETIN in different treatment combinations on direct differentiation of shoots from thalamus ex-plants of French marigold (Tagetes patula L. var. “Pusa Deep”) have been studied. A total of nine treatments was used to screen thalamus ex-plants to determine the capability for plant regeneration and subsequently to find out the optimum medium conditions for high potentiality of direct shoot formation. Thalamus ex-plants behaved differently in all over nine treatments and all the treatments are highly significant over the (T0) control-MS devoid of hormones. The best medium found for direct, shoot organogenesis from the thalamus of Pusa Deep was treatment (T7) MS + BAP 1.0 mg/l + KIN 0.5 mg/l + IAA 1.0 mg/. Pre-treatment with Carbendazim (0.2%) + Mancozeb (0.2%) + 8-HQC (200 mg/l) for 1.5hr resulted in minimum fungal (28.33%), bacterial (25.33%) contamination. Surface sterilization with 0.1% HgCl2 for 5 min reduced microbial contamination up to (44.33%) simultaneously increased the survival percentage upto (55.67%). This technique can be utilized in gene transfer technology, in vitro mutagenesis, production of lutein rich pharmaceutical compounds and secondary metabolites using tissue culture techniques.

Pages: 373-377  |  1053 Views  410 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Soudamani Karjee, Namita, Kanwar P Singh and Sapna Panwar. Development of in-vitro protocol for direct regeneration from thalamus ex-plant of Tagetes patula L. var. Pusa Deep. J Pharmacogn Phytochem 2020;9(4):373-377. DOI: 10.22271/phyto.2020.v9.i4e.11716

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