Author(s):

Misal DR, Kharde AV, Deshmukh VD and Talekar BK

Abstract: Micropropagation technique is used for the elicitation of bacoside content in Bacopa monnieri. In vitro propagation was carried out on Murashige and Skoogs medium supplemented with 6-benzylaminopurine (1.1 µM) and Indole-3- butyric acid (0.30 µM) with 3% sucrose for four weeks. For elicitation variable concentrations of Aspergillus niger filtrate (0, 0.5, 1.0, 1.5, 2.0 ml/L) were incorporated in MS basal medium for 7 days. Present investigation clearly indicated that the Aspergillus niger filtrate significantly influenced the Bacoside production in Brahmi. After seven days elicitation, maximum enhancement in Bacoside (1.62% DW) was reported at lower concentrations of Aspergillus niger filtrate (0.5 ml/L) over control.

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