Journal of Pharmacognosy and Phytochemistry
Vol. 9, Issue 6 (2020)
Isolation, characterization and identification of endophytic bacteria of Hordeum vulgare by molecular sequencing technique
Author(s):
Aisha Parween and Dr. Vidyanath Jha
Abstract:
Bacterial endophytes are ubiquitous colonizers of the inner plant tissues where they do not normally cause any substantial morphological changes and disease symptoms. In this paper we will give an overview of which bacterial species can live as endophytes, and how they enter a plant and live inside. We will also describe various bacterial traits which are required for a successful colonization of the plant’s interior by endophytes. Some endophytes can promote plant growth and/or protect their host against phytopathogens. Many mechanisms of their beneficial action are predicted, but we will focus on those for which experimental support
in planta was reported. Endophytic bacteria live symbiotically with the plant and in turn helping the plant in number of ways. The present investigations were undertaken to isolate and identify bacterial endophytes in roots of
Hordeum vulgare. A total of two endophytic bacteria were isolated from the parts of the plant. On the basis of the morphological and biochemical characterization of the endophytes as well as 16S rRNA sequencing technique Studies on the optimization of growth of the isolates were performed by varying pH & temperature conditions. The ability of bacterial isolates was tested for different enzyme activity and also screened for sensitivity of different antibiotics.
Pages: 2022-2024 | 731 Views 291 Downloads
Aisha Parween and Dr. Vidyanath Jha. Isolation, characterization and identification of endophytic bacteria of Hordeum vulgare by molecular sequencing technique. J Pharmacogn Phytochem 2020;9(6):2022-2024.