Author(s):

VR Bangar, SV Kolase, SB Sable and SB Latake

Abstract: The total 17 fungicide degrading isolated strains (FDB1 to FDB17) were tested for fungicide degrading potential. All the strains were able to utilize the sole carbon source from fungicides viz., difenoconazole, myclobutanil and fluopyram+ tebuconazole at different concentrations for its growth and development but all strains showed variation in their tolerance level of fungicide. In mineral salt agar medium spiked with difenoconazole at 0.5 ml/L, myclobutanil (0.4 g/L) and fluopyram+ tebuconazole (0.5 ml/L), the highest degree of tolerance was visually observed in FDB5, FDB8, FDB13 and FDB16 strains which exhibited moderate growth. In another experiment, all the bacterial strains exhibited the growth in MSM amended with either fungicide or glucose as a sole carbon source throughout the period of study. All the strains were able to utilize a sole carbon source from fungicides as compared with glucose. The turbidity of inoculated broth was increased day by day and recorded as in absorbance. Among all the strains, the bacterial strains viz., FDB5, FDB8, FDB13 and FDB16 were showed maximum absorbance on 2 days of incubation and it was approximately near about the absorbance of glucose amended medium. The results indicated that, the absorbance was recorded on 0 days of incubation was approximately ~1.5 × 10⁸ CFU/ml counts as compared with McFarland standard. After 1 day of incubation, the absorbance was increase and their count was approximately ~3×10⁸ CFU/ml. More than 3×10⁸ CFU/ml population was recorded on 2^nd day of incubation. It was found that all the fungicide degrading strains had the capability to break down each of the three triazole fungicides and was utilized the carbon for their growth. These fungicide degrading strains were sent to the National Centre for Microbial Resources, Pune for identification. These strains were identified on the basis of 16S rRNA gene sequencing 1200 bp and the results obtained are as; FDB5 (Lysinibacillus macrolides), FDB8 (Inquilinus limosus), FDB13 (Stenotrophomonas acidaminiphila) and FDB16 (Bacillus subtilis).

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