Author(s):

Serm Surapinit, Ratchaniporn Kongsui, Napatr Sriraksa, Sitthisak Thongrong and Suttira Sedlak

Abstract: The rhizomes of Boesenbergia rotunda, Zingiber purpureum, and Alpinia galangal are well-known Thai medicinal plants has been used as spice and folk medicine due to its numerous functional and nutritional properties. The present study, the anti-platelet aggregation activities of the ethanol extracts from the rhizomes of rhizomes of B. rotunda, Z. purpureum, and A. galangal were evaluated using 96-well plate platelet aggregation assay. The ethanol extracts from B. rotunda, Z. purpureum, A. galanga (IC50 0.064 ± 0.039, 0.019±0.721, and 0.113±0.031 µg/mL) were more active than the ibuprofen (IC50 0.439 ± 0.064 µg/mL) in ADP-induced platelet aggregation (P<0.05). The inhibitory activity of extract from Z. purpureum (IC50 0.024±0.08 µg/mL) was comparably active to the ibuprofen (IC50 0.036±0.006 µg/mL) in the AA induced platelet aggregation; however, the extracts from B. rotunda and A. galangal (IC50 0.054±0.031 and 0.068±0.026 µg/mL) were less potent than ibuprofen (P<0.05).The ethanol extracts from B. rotunda and Z. purpureum (IC50 0.025±0.008 and 0.012±0.015 µg/mL) were more potent than ibuprofen (IC50 0.056±0.023 µg/mL) in collagen-induced platelet aggregation (P<0.05). The extracts from Z. purpureum and A. galanga (IC50 225±0.094 and 190±0.075 µg/mL) possessed inhibitory activities on thrombin-induced platelet aggregation. The ethanol extracts from the rhizomes of rhizomes of B. rotunda, Z. purpureum, and A. galangal were inactive in the platelet aggregation assay using U-46619 and TRAP-6 as agonists.

Pages: 27-31  |  1446 Views  518 Downloads

Download (733KB)