Author(s):

Kajal Mane, Damayanti Guldekar, SR Potdukhe, BD Sonune, VS Wargane, VS Gavade, Hemalata Khobragade and VD Pawar

Abstract: An investigation entitled “Comparison of different oil formulations on shelf life of Trichoderma asperellum” was carried out during 2018-2019. The laboratory experiment was carried out in Completely Randomized Design with nine treatment in three replication. Paraffin oil, soybean oil, groundnut oil, potato dextrose broth and talc powder were extensively used as carrier for Trichoderma asperellum a at an intervals of 30, 60, 90, 120, 150 and 180 DAI. It was revealed from fshelf life studies that there were significant differences at all the intervals over uninoculated control. Maximum CFU count was recorded with the treatment Paraffin oil 28 x 10⁸ CFU at 30 DAS and it was significantly superior over all other treatment except Talc based culture 27.33 x 10⁸ CFU and was gradually increased upto 90 DAS and thereafter found to be declined from 120 to 180 DAI in all the treatments. It was further noticed that maximum spore germination per cent was noticed in Paraffin oil 59.18 percent at 30 DAT followed by Talc based culture 54.06 percent. There were significantly reduction in spore germination from 60 DAI till 180 DAI in all the treatment. As regards of percent growth inhibition at 8^TH DAI, among three plant pathogenic fungi F. oxysporum inhibited maximum growth 74.44 per cent followed by R. bataticola 81.85 per cent and S. rolfsii in paraffin oil treatment.

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