Journal of Pharmacognosy and Phytochemistry
Vol. 10, Issue 1 (2021)
Standardizing efficient in vitro surface sterilization protocol for explants of Kankoda (Momordica dioica Roxb.)
Author(s):
SS Dodake, AS Jadhav, SV Pawar, PY Shinde and NM Magar
Abstract:
To standardize the sterilization procedure for leaf and apical bud explants of Kankoda (
Momordica dioica Roxb.), comparison was done between two important sterilizing agents, mercuric chloride (0.1%) and sodium hypochlorite (1.0%) with four time duration 3, 5, 7 and 10 minutes and in combinations. Result showed that amongst the two sterilants i.e. HgCl
2 and NaOCl in combination was found better for controlling the infection. Mercuric chloride (HgCl
2) for 5 minute and sodium hypochlorite (NaOCl) for 5 minute in combination was selected for suitable sterilization agents after 10 minute of DDW wash, 10 minute dip in bavistin and at last washed with double distilled water for leaf explants with 75.00% survival and apical bud explants with 83.33% survival of explants. This is the first report of successful sterilization and reduced contamination of explants from naturally field grown Kankoda by using HgCl
2 and NaOCl sterilizing agents in combination for standardizing efficient
in vitro surface sterilization protocol.
Pages: 621-626 | 1804 Views 987 Downloads
SS Dodake, AS Jadhav, SV Pawar, PY Shinde and NM Magar. Standardizing efficient in vitro surface sterilization protocol for explants of Kankoda (Momordica dioica Roxb.). J Pharmacogn Phytochem 2021;10(1):621-626.