Author(s):

Mukesh and Sharma SK

Abstract: The mycelail growth of Cercospora punicae on PDA is very slow therefore the present investigation describes the effects of media, pH, temperature and standardized the best media for qucik growth of Cercospora punicae (leaf and fruit spot disease of pomegranate) in vitro. Five media, Potato Dextrose Agar (PDA), Czapeck Dox Agar (CDA), Green Pomegranate Leaf Decoction Agar 5% (GPLDA), Oat Meal Agar (OMA) and Vegetable Juice Agar (V-8JA) were evaluated for their effects on growth of the pathogen. Green Pomegranate Leaf Decoction Agar medium 5% (57.50 mm) was found to support maximum mycelail growth of fungus in vitro. Therefore, five different concentrations (5; 10; 15; 20; 25%) of Green Pomegranate Leaf Extract in Potato Dextrose Agar were evaluated for standardization of optimum concentration of leaf extract for getting maximum growth of Cercospora punicae in minimum possible time. The maximum radial growth of C. punicae (90 mm) was attained at 15 per cent Green Pomegranate Leaf Decoction Agar medium in 18 days and standardized as specific medium for conducting all the laboratory studies. The maximum growth was observed at temperature levels 25 ^oC (89.85 mm) which was significantly superior to all other temperatures levels viz., 15, 20, 30 and 35 0C on GPLDA medium. The pathogen grew best on pH 6 and the least growth occurred at pH 4.

DOI: 10.22271/phyto.2021.v10.i1l.13427

Pages: 816-818  |  1256 Views  648 Downloads

Download (872KB)