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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 10, Issue 1 (2021)

Development of specific culture media, studies on effect of media, pH and temperature on growth of Cercospora punicae, the causal agent of fruit and leaf spot disease of pomegranate

Author(s):

Mukesh and Sharma SK

Abstract:
The mycelail growth of Cercospora punicae on PDA is very slow therefore the present investigation describes the effects of media, pH, temperature and standardized the best media for qucik growth of Cercospora punicae (leaf and fruit spot disease of pomegranate) in vitro. Five media, Potato Dextrose Agar (PDA), Czapeck Dox Agar (CDA), Green Pomegranate Leaf Decoction Agar 5% (GPLDA), Oat Meal Agar (OMA) and Vegetable Juice Agar (V-8JA) were evaluated for their effects on growth of the pathogen. Green Pomegranate Leaf Decoction Agar medium 5% (57.50 mm) was found to support maximum mycelail growth of fungus in vitro. Therefore, five different concentrations (5; 10; 15; 20; 25%) of Green Pomegranate Leaf Extract in Potato Dextrose Agar were evaluated for standardization of optimum concentration of leaf extract for getting maximum growth of Cercospora punicae in minimum possible time. The maximum radial growth of C. punicae (90 mm) was attained at 15 per cent Green Pomegranate Leaf Decoction Agar medium in 18 days and standardized as specific medium for conducting all the laboratory studies. The maximum growth was observed at temperature levels 25 oC (89.85 mm) which was significantly superior to all other temperatures levels viz., 15, 20, 30 and 35 0C on GPLDA medium. The pathogen grew best on pH 6 and the least growth occurred at pH 4.

Pages: 816-818  |  1129 Views  581 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Mukesh and Sharma SK. Development of specific culture media, studies on effect of media, pH and temperature on growth of Cercospora punicae, the causal agent of fruit and leaf spot disease of pomegranate. J Pharmacogn Phytochem 2021;10(1):816-818. DOI: 10.22271/phyto.2021.v10.i1l.13427

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