Author(s):

Nikhil Sharma and Khushboo Kathayat

Abstract: Plant tissue culture is commonly used in the field of horticulture, agriculture, plant breeding and forestry. It is applied form of biotechnology employed for propagating plants in large quantity, elimination of virus, manufacturing of secondary metabolites and aseptic cloning of plants. Tissue culture in plants has been also employed for the protection of extinct species of plants by short and medium term protection also called as slow growth and cryopreservation also termed as long term conservation. These practices had been successfully employed to protect plant species with recalcitrant seeds or dormant seeds and exhibit considerable edge over the traditional methods of conservation.

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