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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 10, Issue 1 (2021)

In vitro assessment of quantitative analysis of phytochemicals, iron (II) chelating activity and antimicrobial efficacy of methanolic solvent extract of citrus maxima leaf

Author(s):

Nishat Akther, Rahaman M Abdur, Hossain M Amjad, Rahman M Foysol, Shamima Afroze, Ferdaushe Akter, Islam M Johirul and Md. Nazmul Hossain

Abstract:
Herein, we report the quantitative analysis of phytochemicals, iron (II) chelating activity, and antimicrobial efficacy of Citrus maxima (Pomelo) leaves in methanol extract using Folin-Ciocalteu’s colorimetric assay, aluminium chloride colorimetric assay, Ferrous-ferrozine complex inhibition assay and agar disc diffusion method. The total phenol contents extracted as 117.68±0.63 mg of GAE/g of dry extract and 14.39±0.09 mg of CE/g of dry extract and for total flavonoid it was 28.55±0.39598 mg of CE/g of dry extract. The inhibitory concentration (IC50) of ferrous ion chelating activity was recorded as 323.79 μg/mL. Also, this chelating activity increases with the extract concentrations. On the other hand, this leaf extracts displaced a concentration dependent inhibition of microorganisms, the eleven different cultures responded to samples, by virtue of which we obtained in the range of 5.5-12 mm zone of inhibition. Especially, this leaf extract shows a satisfactory antibacterial efficacy on Salmonella paratyphi, Salmonella typhi and V. cholerae C6706. The outcomes of the study corroborate the extract samples, and can even be a brand-new source for several drug discoveries.

Pages: 1988-1998  |  869 Views  298 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Nishat Akther, Rahaman M Abdur, Hossain M Amjad, Rahman M Foysol, Shamima Afroze, Ferdaushe Akter, Islam M Johirul and Md. Nazmul Hossain. In vitro assessment of quantitative analysis of phytochemicals, iron (II) chelating activity and antimicrobial efficacy of methanolic solvent extract of citrus maxima leaf. J Pharmacogn Phytochem 2021;10(1):1988-1998.

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