Author(s):

Sameena Lone, K Hussain, S Narayan, Nawaz Ahmad, Majid Rashid, Syed Mazahir Hussain, Harish Kumar and Zahedullah Zahid

Abstract: The present work aimed to determine the pollen viability of ten minicore accessions of cherry tomato (Solanum spp.) was carried out at the Experimental Farm, Division of Vegetable Science, SKUAST-K, Shalimar, India during Kharif season 2020. The freshly opened male flower buds were collected in the morning between 7.30 am to 8.00 am. TZ test also known as Tetrazolium (2, 3, 5 triphenyl tetrazolium chloride) test was followed to determine the pollen viability of ten minicore accessions of cherry tomato. All living tissues, which respire, possess the ability of reducing a colour less chemical 2, 3, 5 triphenyl tetrazolium chloride into a red coloured compound formazan by H transfer reactions catalysed by the enzyme dehydrogenases (Norton, 1966). This method provides accurate results for many taxa as compared to other methods of staining (Shivanna and Johri, 1989) ^[30]. The concentration of tetrazolium salt, temperature and period of incubation needs to be standardized to get optimal and precise results in various pollen samples (Shivanna and Johri 1989) ^[30]. Based on the overall performance of the accessions, the maximum pollen viability percentage was showed by accession EC-520078 followed by VRT-02 and the least was showed by accession Suncherry followed by WIR-5032.

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