Journal of Pharmacognosy and Phytochemistry
Vol. 10, Issue 2 (2021)
In vitro antioxidant activities of the roots of Coffea benghalensis B Heyne ex Schult. Growing in Bangladesh
Rifat Ara Rima, Shoriful Islam Sagor and Adeeba Anjum
In the present study, antioxidant potential of the crude methanol extract along with its n
-hexane, chloroform, ethyl acetate and aqueous soluble fractions of the roots ofCoffeabenghalensis
growing in Bangladesh was assessed by usingDPPHscavenging assay, reducing power, hydroxyl radical scavenging assay and total antioxidant capacity.In DPPH scavenging assay the IC50
value of the ethyl acetate soluble fraction was found to be 47.56 µg/ml while the reference standard ascorbic acid was 1.42 µg/ml. The reducing power showed good linear relations between the standard ascorbic acid and all the test samples. Higher hydroxyl radical activity was shown by the n
-hexane soluble fraction with IC50
value 58.58 µg/mlwhereas the standard catechin was 89.61 µg/ml. The extracts showed significant activities in total the antioxidant assaycompared to the reference standard in a dose dependent manner. Also total phenolic and flavonoid content was evaluated which exhibited remarkable values.
Pages: 40-45 | 486 Views 176 Downloads
Rifat Ara Rima, Shoriful Islam Sagor and Adeeba Anjum. In vitro antioxidant activities of the roots of Coffea benghalensis B Heyne ex Schult. Growing in Bangladesh. J Pharmacogn Phytochem 2021;10(2):40-45.