Author(s):

Koledoye O Funmilayo, Olaleye M Tolulope, Akinmoladun A Clement, Crown O Olamide and Lawal A Olalekan

Abstract: Phytochemicals present in Luffa cylindrica (L.) M. Roem plant may be responsible for its cytotoxicity activity against cancer cell lines. The present study investigated the phytoconstituents present in Luffa cylindrica methanolic leaf extract and evaluates its cytotoxicity activity on colorectal cell lines using MTT assay method. Gas chromatography- mass spectrometry (GC-MS) and High performance liquid chromatography (HPLC) methods were used to identify the phytochemical constituents of Luffa cylindrica methanol leaf extract. GC-MS analysis result revealed the presence of various volatile organic compounds ranging from alcohols, triterpenes, phenols, fatty acids, esters, and steroids. A total number of 18 compounds were identified with GC-MS while HPLC analysis identified 12 different phytochemicals, some of which are known to possess cytotoxicity activities both in vitro and in vivo. The plant extract showed a dose dependent cytotoxicity activity against the tested cell lines. The result from this finding shows that methanol leaf extract of Luffa cylindrica possess anticancer properties which could be further investigated for the development of cancer therapy.

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