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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 10, Issue 2 (2021)

Inhibitory effect of piperine on AcrAB-TolC efflux pump in fluroquinolone resistant Escherichia coli isolates from bovine mastitis milk samples

Author(s):

Vanishree, Nisha AR, Sanis Juliet, Suja Rani S and Naicy Thomas

Abstract:
‘Antimicrobial resistance’ is a global epidemic issue causing economic loss to dairy farmers especially when treatment become unresponsive to mastitis. In the recent past, role of efflux pumps in development of resistance is intervened so as to overcome bacterial resistance to broad class of chemotherapeutic agents. In the current study an attempt has been made to investigate the ability of ‘piperine’, a phytochemical to overcome the efflux pump, AcrAB-TolC mediated resistance in E. coli isolates from bovine mastitis. The study revealed piperine reduced the MIC of enrofloxacin and ciprofloxacin against E.coli isolates from mastitis and showed synergistic action at a concentration of 100 ppm. A 16 fold reduction in MIC of enrofloxacin and ciprofloxacin were observed upon co-exposure at concentration of 100 ppm. Gene expression studies indicated down regulation of expression of acr A, acr B and tol C efflux pump in E. coli isolates resistant to enrofloxacin and ciprofloxacin. Piperine showed increased efflux pump inhibitory activity in ciprofloxacin resistant E. coli isolates. In silico analysis revealed that piperine binds with acrB protein with least binding energy. This study revealed that piperine potentiated the action of enrofloxacin and ciprofloxacin by inhibition of AcrAB-TolC efflux pump.

Pages: 319-327  |  1140 Views  298 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Vanishree, Nisha AR, Sanis Juliet, Suja Rani S and Naicy Thomas. Inhibitory effect of piperine on AcrAB-TolC efflux pump in fluroquinolone resistant Escherichia coli isolates from bovine mastitis milk samples. J Pharmacogn Phytochem 2021;10(2):319-327.

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