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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 10, Issue 2 (2021)

Standardization of in vitro rooting and ex vitro hardening in traditional banana cultivars of Kovvur Bontha (ABB) and TellaChakkeraKeli (AAA)

Author(s):

CR Sushma, K Ravindra Kumar, R Naga Lakshmi, P Rama Devi, P Vinaya Kumar Reddy, G Ramanandam and T Gowri Shankar

Abstract:
Banana is most important crop in India and fetching more foreign money in international trade. Banana and plantain are important staple foods that are critical to nutritious and economic well-being of millions of people across the globe. Banana cv. TellaChakkeraKeli (AAA) is choicest table variety in Andhra Pradesh and popular in Godavari districts. Banana cv. Kovvur bontha (ABB) is highly popular in AP and other southern districts mostly used for culinary purpose. Disease free high yielding planting is major constraint for commercial production of both these native genotypes. Poor in vitro root induction and ex vitro survival are the major hindrance for its large scale commercial production. Therefore, the objective of the present investigation was to develop an efficient culture media for in vitro rooting and ex vitro performance of both the genotypes. Among the different treatments evaluated, micro-shoots cultured on ½ MS media supplemented with two kinds of auxins (NAA and IBA at lower concentration) were proved to be good in terms of highest root induction in banana cv. TellaChakkerakeli and Kovvur Bontha. Among the different treatments, shoots cultured on ½ MS media supplemented with 0.5 mg l-1 NAA gave 100 % rooting, increased girth (2.23, 2.01 mm), pseudostem height (6.09, 5.77 cm), leaf area (7.60, 7.53 cm2) and no. of leaves per shoot (5.60, 5.23) in both TellaChakkerakeli and Kovvur Bontha respectively. Under ex vitro hardening 100 % survival was obtained on the ½ MS media supplemented with 0.5 mg l-1 NAA (for cv. TellaChakkerakeli) and on 0.5 mg l-1 IBA (for cv. Kovvur Bontha) which are proved to be the best treatments accordingly for most of the characters in both the cultivars.

Pages: 688-693  |  745 Views  336 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
CR Sushma, K Ravindra Kumar, R Naga Lakshmi, P Rama Devi, P Vinaya Kumar Reddy, G Ramanandam and T Gowri Shankar. Standardization of in vitro rooting and ex vitro hardening in traditional banana cultivars of Kovvur Bontha (ABB) and TellaChakkeraKeli (AAA). J Pharmacogn Phytochem 2021;10(2):688-693.

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